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sinv nsp3  (Biotium)


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    Structured Review

    Biotium sinv nsp3
    Lack of E2 envelope glycoprotein on the surface of some <t>SINV-infected</t> cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV <t>nsP3</t> (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.
    Sinv Nsp3, supplied by Biotium, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sinv+nsp3/pmc07093546-123-10-19?v=Biotium
    Average 86 stars, based on 1 article reviews
    sinv nsp3 - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs"

    Article Title: Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-61015-0

    Lack of E2 envelope glycoprotein on the surface of some SINV-infected cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV nsP3 (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.
    Figure Legend Snippet: Lack of E2 envelope glycoprotein on the surface of some SINV-infected cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV nsP3 (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.

    Techniques Used: Infection, Staining, Membrane



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    Lack of E2 envelope glycoprotein on the surface of some <t>SINV-infected</t> cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV <t>nsP3</t> (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.
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    Lack of E2 envelope glycoprotein on the surface of some <t>SINV-infected</t> cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV <t>nsP3</t> (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.
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    Image Search Results


    Lack of E2 envelope glycoprotein on the surface of some SINV-infected cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV nsP3 (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.

    Journal: Scientific Reports

    Article Title: Visualization of cell-type dependent effects of anti-E2 antibody and interferon-gamma treatments on localization and expression of Broccoli aptamer-tagged alphavirus RNAs

    doi: 10.1038/s41598-020-61015-0

    Figure Lengend Snippet: Lack of E2 envelope glycoprotein on the surface of some SINV-infected cells. dAP7 cells infected with SINV Toto E2-mCherry (MOI = 20), followed by treatment with anti-E2 [SV127, 5 μg/ml] 2 h after infection were fixed with 3.7% formaldehyde at 24 h were stained with anti-SINV nsP3 (white) and anti-mouse IgG (green). The viral E2 glycoprotein (red) is on the membrane of the cell that antibody (green) can bind (white arrows). In contrast, antibody cannot bind another cell that has only intracellular E2 (red arrows). Images are representative of multiple cells in the culture with similar staining. Scale bar = 100 μm.

    Article Snippet: Infected cells were identified with a rabbit polyclonal antibody to SINV nsP3 and CF633-conjugated anti rabbit IgG(H + L) (Biotium).

    Techniques: Infection, Staining, Membrane